Abstract: OBJECTIVE To establish the optimum preparative procedure for ultrasonic extracting bergenin from Rodgersia aesculifolia Batal. METHODS The optimum conditions of extraction process for bergenin from Rodgersia aesculifolia Batal were selected with the extractive yield of bergenin as marks by orthogonal experimental design, investigating the ratio of extracting temperature, solid to liquid, extracting power, extracting time and extracting times. RESULTS The result showed that the most important factors were extracting times, extracting time and solid to liquid. The optimum preparative procedure was as follows: Rodgersia aesculifolia Batal was extracted with ultrasonic technology at solid-liquid ration of 1∶14(g·mL-1), 60 W, 70 ℃ and 60 min for four times. CONCLUSION The process is feasible and useful for extracting bergenin from Rodgersia aesculifolia Batal, with average extracting rate of bergenin as 12.588 5 mg·g-1.