HPLC测定地耳草中槲皮苷的含量

Determination of Quercitrin in Hypericum japonicum Thunb. By HPLC

摘要: 目的测定地耳草中槲皮苷的含量。方法采用高效液相色谱法，Agilent C18柱(250 mm×4.6 mm, 5 μm)，流动相为乙腈-0.05 mol·L-1磷酸二氢钾溶液(19∶81)，检测波长为256 nm，流速为1.0 mL·min-1。结果槲皮苷进样量在0.120 4 ～2.408 μg内呈良好的线性关系，平均回收率为96.0％，RSD=1.3%(n=6)。结论该方法快速简便，稳定可靠，专属性强。

Abstract: OBJECTIVE To determine the content of quercitrin in Hypericum japonicum Thunb. By HPLC. METHODS An Agilent C18 column(250 mm×4.6 mm, 5 μm) was used. The mobile was acetonitrile-0.05 mol·L-1 potassium dihydrogen phosphate(19∶81), and detected at 256 nm with flow rate of 1.0 mL·min-1. RESULTS A good linearity was observed for quercitrin in the range of 0.120 4-2.408 μg. The average recovery of quercitrin was 96.0% with RSD of 1.3%(n=6). CONCLUSION The method is convenient, stable, reliable and special for the dermination of quercitrin in Hypericum japonicum Thunb.