Abstract: OBJECTIVE To study the targeted properties of dexamethasone acetate solid lipid nanoparticles in mice. METHODS A high performance liquid chromatographic (HPLC) assay for the determination of dexamethasone acetate in various tissues of mouse was employed in this study. Targeting properties were evaluated by comparing dexamethasone acetate content, percentage of dexamethasone acetate in various organs at different time, and pharmacokinetics parameters after administration of dexamethasone acetate solid lipid nanoparticles with those after administration of active compound. RESULTS Dexamethasone acetate content, percentage of dexamethasone acetate in lung were higher for the dexamethasone acetate - SLN group than that for the dexamethasone acetate - propylene glycol solution group. The difference of AUC between the two groups is 16 times. CONCLUTION Dexamethasone acetate solid lipid nanoparticles have targeting properties for lung in mice.