HPLC同时测定银黄颗粒中绿原酸和黄芩苷的含量

Simultaneously Determination of Chlorogenic Acid and Baicalin in Yinhuang Granules by HPLC

摘要: 目的建立同时测定银黄颗粒中绿原酸和黄芩苷含量的高效液相色谱方法。方法Agilent Eclipse XDB-C18色谱柱(4.6 mm ×150 mm, 5 μm)；柱温为30 ℃；流动相为0.1%磷酸(A) -乙腈 (B)；流速为1. 0 mL·min-1；梯度洗脱；检测波长为327 nm。结果绿原酸在0.021～2.1 μg内线性关系良好(r=0.999 8)；黄芩苷在0.038～3.800 μg内线性关系良好(r=0.999 6)。绿原酸、黄芩苷平均加样回收率为99.70%，99.06%，RSD分别为0.75%和1.02%。结论所建立的方法简便、准确可以同时测定银黄颗粒中绿原酸和黄芩苷的含量。

Abstract: OBJECTIVE To develop an HPLC method for the simultaneously determination of chlorogenic acid and baicalin in Yinhuang granules. METHODS HPLC was carried out, using Agilent Eclipse XDB-C18 (4. 6 mm ×150 mm, 5 μm) column set at 30 ℃, the acetonitrile-0.1% H3PO4 as mobile phase(using gradient) with flowing rate of 1.0 mL·min-1. The detection wavelength was set at 327 nm. RESULTS The method had good linear relationship within the range of 0.021-2.1 μg (r =0.999 8) for chlorogenic acid and 0.038-3.800 μg (r =0.999 6) for baicalin,respectively. The results of the average recovery were 99.70% (RSD=0.75%) and 99.06% (RSD=1.02%). CONCLUSION The method established was simple and accurate and can be used to simultaneously determine chlorogenic acid and baicalin in Yinhuang granules.