HPLC同时测定疗筋涂膜剂中5种大黄成分的含量

唐洪梅; 李得堂; 闫雪; 丘振文; 蔡庆群

HPLC同时测定疗筋涂膜剂中5种大黄成分的含量

Simultaneous Determination 5 Contents of the Rhubarb in the Liaojin Film by HPLC

摘要

摘要: 目的建立高效液相色谱法同时测定疗筋涂膜剂中5种成分的含量。方法采用Hypersil ODS-C18柱(250 mm × 4.6 mm，5 μm) , 甲醇-0.05％磷酸(85∶15)为流动相，流速：0.8 mL·min-1，柱温：34 ℃，检测波长：254 nm。结果芦荟大黄素在9.11～145.76 μg·mL-1（r=0.999 4），大黄酸在5.63～90.08 μg·mL-1（r=0.999 3），大黄素在5.44～87.04 μg·mL-1（r=0.999 9），大黄酚在6.13～98.08 μg·mL-1(r=0.999 9)，大黄素甲醚在2.72～43.52 μg·mL-1 (r=0.999 8)线性良好；平均回收率分别为97.64%、98.14%、99.52%、99.36%、98.43%；RSD分别为1.29%、1.81%、1.40%、0.59%、1.58%。结论该方法简便、可靠、准确，可用于疗筋涂膜剂的质量控制。

Abstract: OBJECTIVE To establish an HPLC method for the determination of Chrysophanol, Emodin, Physcion, Aloeemodin and in Liaojin film．METHODS The determination was performed on Hypersil ODS-C18 column(250 mm x 4.6 mm，5 μm)with mobile phase consisted of methanol-0．05％phosphoric acid (85∶15)at a flow rate of 0.8 mL·min-1．The UV detection wavelength was set at 254 nm．RESULTS The calibration curves were linear in the range of 9.11-145.76 μg·mL-1（r=0.999 4）for Aloeemodin，5.63-90.08 μg·mL-1（r=0.999 3）for Rhein，of 5.44-87.04 μg·mL-1（r=0.999 9）for Emodin，6.13-98.08 μg·mL-1 (r=0.999 9)for Chrysophanol and 2.72-43.52 μg﹒mL-1(r=0.999 8) for Physcion. The average recovery was 97.64%，98.14%，99.52%，99.36%，98.43%；RSD was1.29%，1.81%，1.40%，0.59%，1.58%.CONCLUSION The determination method is simple，reliable，accurate, and it can be used for quality control of Liaojin film．

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