体外人胃癌细胞凋亡试验优选黄参膏提取工艺

Optimization of the Extraction Process of Huangshen Electuary by Apoptosis of Human Gastric Cancer Cells in Vitro

摘要: 目的优选黄参膏的最佳提取工艺。方法选择提取次数、提取时间、溶剂倍数为考察因素，各因素均选3个水平，以L9(34)正交试验法制备黄参膏，以体外人胃癌细胞凋亡指数作为工艺考察指标，优选黄参膏的最佳提取工艺。结果最佳提取工艺为加8倍药材量水，煮提2次，每次煎煮1 h。结论优选得到的提取工艺稳定、可行，进一步验证诱导胃癌细胞凋亡是黄参膏抗癌作用的机制之一。

Abstract: OBJECTIVE To optimize the extraction technology for Huangshen Electuary. METHODS The effects of three factors including the times of extraction, time length of extraction and solvent ratio were investigated, and each factor was chosen three levels. Optimize extraction of Huangshen Electuary in the L9(34) orthogonal test, as apoptosis of human gastric cancer cells in vireo as an index. RESULTS The best extraction technology is: adopt 8 times quantities of water to boil for 2 times,1 hours for each time. CONCLUSION The optimum extraction technology for Huangshen Electuary is feasibie. The induction of human gastric cancer cells apoptosis is one of Huangshen Electuary effect mechanism.