Abstract: OBJECTIVE To investigate the protective effect of Quercitrin against ulcerative colitis in rats and anti-inflammatory, analgesic and anti-diarrhea effects of Quercitrin in mice. METHODS Rats treated with oral Quercitrin （50,200 mg·kg-1·d-1） for 2 days before and 6 days after ulcerative colitis induced by acetic acid. Colonic pathological changes, tissue malondialdehyde (MDA) and myeloperoxidase (MPO) levels were determined. Anti-inflammatory, analgesic and anti-diarrhea effects of Quercitrin （75,300 mg·kg-1·d-1） treated with oral for 4 days in mice were determined by penetrability of abdominal capillary vessel assay, acetic acid-induced writhing assay and senna-induced diarrhea assay respectively. RESULTS Quercitrin improved colonic pathological changes and reduced MDA and MPO levels in ulcerative colitis rats (P<0.05). Quercitrin suppressed the increased penetrability of abdominal capillary vessel induced by acetic acid (P<0.05); inhibited the writhing response induced by acetic acid (P<0.05); but had no significant effect on diarrhea induced by senna in mice (P>0.05). CONCLUSION Quercitrin has the protective effect against ulcerative colitis in rats and has anti-inflammatory and analgesic effects in mice.