HPLC测定三七花蕾中的皂苷含量研究

Determination of Saponins in the Flower Buds of Panax Notoginseng by HPLC

摘要: 目的建立三七花蕾中8种四环三萜皂苷（人参皂苷Rg1、Re、Rb1、Rc、Rb2、Rb3、Rd和三七皂苷R1）的高效液相色谱定性定量测定方法。方法采用Hypersil ODS（4.6 mm×250 mm，5 μm）分析柱，以含0.005%甲酸的乙腈-水进行梯度洗脱，在65 min内获得良好分离。结果三七花蕾中8种皂苷在一定的浓度范围内，与峰面积呈良好的线性关系，回收率分别为：R1 95.6%～102.5%(n=3), Rg1 98.8%～103.1%(n=3), Re 95.3%～101.7%(n=3)，Rb1 98.9%～101.1%(n=3)，Rc 95.6%～102.3%（n=3），Rb2 99.0%～100.4%（n=3），Rb3 99.2%～100.3%(n=3)，Rd 95.0%～96.8%(n=3)。结论该方法灵敏快速，准确可靠，可用于三七花蕾的质量控制。

Abstract: OBJECTIVE To establish a method for qualitative and quantitative analysis of eight saponins including ginsenosides Rg1, Re, Rb1, Rc, Rb2, Rb3, Rd and notoginsenoside R1 in the flower buds of Panax notoginseng by HPLC. METHODS The analysis was performed on a Hypersil ODS (4.6 mm×250 mm, 5 μm) column with gradient elution of acetonitrile and water both contained 0.005% formic acid in 65 min. RESULTS Eight saponins were detected in samples of flower buds, in which protopanaxadiol saponins were the main active components, and the content of ginsenoside Rb3 was above 3%.CONCLUSION The method was sensitive, rapid and accurate with good reproducibility, and suitable for the quality control of the flower buds of Panax notoginseng.