HPLC测定元胡药材中脱氢延胡索碱的含量

Quantitative Determination of Dehydrocorydaline in Rhizoma Corydalis by HPLC

摘要: 目的建立元胡药材中脱氢延胡索碱含量测定方法。方法色谱柱使用Diamonsil C18 (250×4.6 mm, 5 μm)；乙腈-0.2%醋酸（18∶82）为流动相；检测波长为340 nm；流速1mL·min-1。结果盐酸脱氢延胡索碱在进样量0.163 0～1.630 4 μg内线性关系良好，回归方程为：Y = 1 553.5X+1.306 1，r= 0.999 9；平均回收率为97.9％，RSD为1.38％，n＝9。结论本方法简便，灵敏，准确，重现性好，可用于元胡药材的含量测定和质量控制。

Abstract: OBJECTIVE To determine the content of dehydrocorydaline in Rhizoma Corydalis by HPLC.METHODS The analysis was carried out on Diamonsil C18 (250 mm×4.6 mm, 5 μm).The mobile phase was acetonitrile-0.2% acetic acid (18∶82); wavelength was 340 nm and the flow rate was 1.0 mL·min-1.RESULTS The liner range of dehydrocorydaline was 0.163 0-1.630 4 μg. Regression equation was Y=1 553.5X + 1.306 1，r=0.999 9. The average recovery was 97.9% with RSD=1.38% (n=9).CONCLUSION The methods is accurate with a good reproducibility and can be used as a quantitative analysis for Rhizoma Corydalis.