层析凝胶DEAE Sepharose Fast Flow分离纯化5’-混合脱氧单核苷酸
Purification of 5'-deoxynucleotides by DEAE Sepharose Fast Flow
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Abstract: OBJECTIVE According to the physical and chemical characteristic of 5'-deoxymononucleotides,this study investigated the separating and purifying 5'-deoxymononucleotides by DEAE Sepharose Fast Flow.METHODS Take 20mmol/L Tris(pH 9.0) as binding buffer,sample