重组人内皮抑素在大肠杆菌中表达及其生物学活性鉴定
Expression of recombinant human endostatin in E.coli and identification of its biological activity
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Abstract: OBJECTIVE To express recombinant human endostatin in E.coli and identify its biological activity. METHOD Endostatin cDNA was amplificated by RT PCR from human fresh fetal liver. The synthesized cDNA fragment was cloned into pGEM-T Easy vector. After i
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