反相高效液相色谱法测定血浆中两性霉素B浓度
Determination of amphotericin B in human plasma by reversed-phase HPLC
-
Abstract: OBJECTIVE:To establish a reversed-phase HPLC assay to monitor the plasma concentration of Amphotericin B.METHOD:Amphotericin B was separated on ODS-Hypersil C18 column and detected at UV380nm,using 0.005mol/L EDTA-2Na-methanol(2:8,v/v) as mobile phase,and