双位酶免疫法测定NT-3cDNA-CHO细胞上清中rNT-3含量
Determination of the levels of recombinant neurotrophin-3 in the supernatants of NT-3cDNA/CHO cells by two-site enzyme immunoassay
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Abstract: OBJECTIVE:To determine the levels of recombinant neurotrophin-3(NT-3) in the supernatants of NT-cDNA/CHO cells.METHOD:A two-site enzyme immunoassay(EIA) method was established using the monoclonal antibody 3W3.RESULTS:The limit of detection of the EIA was