Abstract: A RP-HPLC method was established for analysing minocycline. The HPLC conditionswere:200 5 mm ID column containing YWG-C18H37, held at 25+0.5C mobile phase of 0.002mol/L EDTA-Na2 buffer solution (adjust PH=3.0 +0.1 with oxalic acid): meethanol: acetonitri