高浓度葡萄糖注射液细菌内毒素的测定

Detection of Endotoxin in High Concentration Dextrose Injection

摘要: 50%的葡萄糖溶液可抑制鲎试剂凝集反应,干扰细菌内毒素的测定,本文使用中空纤维透析器,以双向超滤技术,将细菌内毒素与葡萄糖分离,降低了葡萄糖浓度,消除了葡萄糖对鲎试剂凝集反应的抑制作用,方法实用,操作简便,快速灵敏。

Abstract: 50% dextrose may inhibit the agglutination reaction of the Tachyleus amebocytelysate reagent and interfere with the detection of endotoxin.In this experiment,we utilized atwo-way ultrafiltration technique with hollow fiber dialyzer and succeeded in separa