多核苷酸磷酸化酶(PNPase)的提取研究

Study of Polynucleotide Phosphorylase (PNPase) Extracted by Lysozyme-Ultrasonication

摘要: 用溶菌酶·超声波法处理大肠杆菌使其破壁。用聚丙烯酰胺凝胶电泳对纯化的PNPase定性,再根据PNPase活力测定及poly Ⅰ的聚合情况,对PNPase的质量进行综合评价。

Abstract: PNPase was extracted by lysozyme-ultrasonication from E. coli. In order to determine the nature and quantity of PNPase, PAGE, activity determination of PNPase and enzymatic synthesis of poly I were carried out. The results indicated that PNPase extracted