Abstract: OBJECTIVE To explore anti-tumor effects of extracts from Ganoderma Lucidum(GLE). METHODS Anticancer effects of GLE were experimented by mice bearing H22 hepatocarcinoma transplantation tumor model in vivo and MTT reduction test in vitro. RESULTS ①GLE could inhibit H22 transplanted tumor growth in mice in a dose-dependent manner, the inhibitive percentage of carcinoma weight was 59.3% in 500 mg·kg^-1 group and there was obviously differentiation when comparing with negative control group(P<0.01). ②GLE had an inhibitory effect on the proliferation of K562 and HL60 human tumor cells and had less inhibitory activity on SMMC7221, HepG2, SW480, SW1116 and SGC7901 human tumor cells. CONCLUSION This study confirms that GLE has significant anticancer effect in vitro and vivo. However, the mechanism and active ingredients remain to be studied.