Abstract: OBJECTIVE To determine the content of jasminoidin, genipin gentiobioside and total iridoid glycoside in 10 batches of Gardenia jasminoides from different areas. METHODS The content of jasminoidin and genipin gentiobioside were determined by HPLC, while total iridoid glycoside by UV. RESULTS The content of iridoid glycoside in Gardenia jasminoides from Jiangxi province performed much higher. CONCLUSION There is difference of the content of iridoid glycosides in Gardenia jasminoides from different areas.