Abstract: OBJECTIVE To study changes of several hydrolase activities in the period of emergence in five years of Ginseng Radix et Rhizoma. METHODS Neutral phosphate buffer was selected to extract crude extract. UV was applied to determine the activities of amylase (AMY), esterase (Est), acid phosphatase (ACP), alkaline phosphatase (ALP), and phytase. RESULTS The trend of Est, ACP, and ALP activities increased first and then decreased to increase. Est activity increased rapidly in the first 7 days, after which there was no significant change in the period of emergence. ACP activity reached a peak on the 10th day of the period of emergence. ALP activity remained steady upward trend. The trend of AMY was similar to phytase in ginseng root. In the first 4-13 days, activity increased rapidly, then decreased. The trend of two enzymes activities was completely opposite in seedlings. CONCLUSION Five hydrolase activity curves are different, which can be used as a physiological index of ginseng seedling morphogenesis at different stages of period of emergence.