正交试验优选雪松松针中莽草酸的提取工艺

Optimization of the Extracting Process of Shikimic Acid from Pine Needles of Cedrus Deodara (Roxb.) G. Don by Orthogonal Experiment

摘要: 目的优选雪松松针中莽草酸的最佳提取工艺。方法采用L9(34)正交试验设计，以莽草酸的含量为指标，以液料比、浸泡时间、提取时间为因素，优化雪松松针中莽草酸的提取工艺。结果最佳提取工艺为加20倍量的水，浸泡0.5 h，加热回流2 h。在最佳工艺条件下，提取的莽草酸含量为3.58%。结论优选得到的提取工艺简单、稳定、可行。

Abstract: OBJECTIVE To optimize the extraction technology and condition of shikimic acid from pine needles of Cedrus deodara (Roxb.) G. Don. METHODS The extraction technology of shikimic acid was optimized by L₉(3⁴) orthogonal experiment with the contents of shikimic acid as index, and with the ratio of raw material to liquid, soaking time and extraction time as factors. RESULTS The optimal extracting process was adding 20 times of water, soaking 0.5 h, reflux 2 h. Under the conditions, the content of shikimic acid was 3.58%. CONCLUSION The optimal extracting process is simple, stable and practical.