Abstract:
OBJECTIVE To establish the method of quality assessment and identification of GC fingerprints for Litsae Frctus.
METHODS The essential oil from 14 batches of Litsae Frctus was extracted by steam distillation, and determined by GC. The measurement conditions applied were as follows: DB-Wax capillary column (30 m×0.25 mm, 0.25 μm), FID detector, inlet temperature was 250 ℃, the detector temperature was 250 ℃, and temperature of column was increased linearly from 50 ℃ to 75 ℃ at a speed of 3 ℃·min
-1, from 75 ℃ to 130℃ at a speed of 10 ℃·min
-1, from 130 ℃ to 240 ℃ at a speed of 5 ℃·min
-1 (15 min). Carrier gas was nitrogen(1.0 mL·min
-1). Split ratio was 10∶1. Volume was 1 μL.
RESULTS The 14 samples were classified by 2 grades according to cluster analysis and the 12 superior in producing area samples were selected to establish the mutual models. The results of similarity analysis showed that the grade Ⅰ was commendatory and the grade Ⅱ was general.
CONCLUSION This method is simple, quick and specific. It is reliable to evaluate the quality of Litsae Frctus.