Abstract: OBJECTIVE To investigate the specificity of interleukin-2 (IL-2) targeting liposomes (L) containing 5 ′ -palmitoyl- 5-fluorouridine (5-FURP). METHODS Liposomes containing 5-FURP were prepared by reverse phase evaporation method. IL-2 targeting 5-FURP-L were prepared through the effect of cross linker. The entrapment efficiency was measured by UV method. The amount of IL-2 conjugated to liposomes was determined with coomassie brilliant blue binding assay. Cytotoxicity on Hut-102 cells expressing IL-2 receptors in vitro was evaluated with MTT methods. RESULTS The mean size of the liposomes was 180 nm and the entrapment efficiency was 91.3%. The coupling efficiency of IL-2 was 56.0%. In pH 7.4 PBS , 5-FURP was released in a sustained manner from the liposomes. The MTT assay suggested that the IL-2 targeting 5-FURP-L had a distinct killing effect on Hut-102 tumor cells. CONCLUSION The IL-2 modified 5-FURP-L has an active targeting function and a significant anticancer effect.