Abstract: OBJECTIVE To explore effects of puerarin on lipidemia, blood coagulation and platelet aggregation in experimental hyperlipidemia rats. METHODS Experimental hyperlipidemia rats were ip administration of puerarin (50 mg·kg^-1·d^-1) or vehicle (0.9% NaCl) in 30 d. The levels of plasma triplyceride (TG), total cholesterol (TC), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), malondialdehyde (MDA), superoxide dismutase (SOD), prothrombin time (PT), activated partial thrombplastin time (APTT), and platelet maximal aggregation rate(MAR) which was induced by ADP in 5 min were measured. RESULTS Plasma TG, TC, LDL-C and HDL-C of the rats fed high fat diet (HFD) and ip puerarin (50 mg·kg^-1·d^-1) were 27%, 21%, 39% lower and 24% higher than that of HFD group rats. The PT and APTT of puerarin and HFD group rats were significantly longer than that of the HFD group (P<0.05). The SOD in puerarin and HFD group was higher (P<0.05) and the MDA and MAR in puerarin and HFD group was lower than that of HFD group (P<0.01). CONCLUSION Puerarin can reduce the serum lipid level of the hyperlipidemia rats effectively and prevent the lipid peroxidation, moreover blood clotting system in hyperlipidemia rats is improved effectively.