马鞭草药材的质量标准研究

舒积成; 侴桂新; 王峥涛

马鞭草药材的质量标准研究

Quality Control of Verbenae Herba

摘要

摘要: 目的建立马鞭草药材的定性、定量标准。方法采用薄层色谱法鉴别马鞭草药材中马鞭草苷、戟叶马鞭草苷和毛蕊花苷成分；以高效液相色谱法测定以上3种成分的含量。色谱柱为Agilent Eclipse XDB C₁₈(4.6 mm×250 mm，5 μm)，流动相为乙腈-甲醇-水梯度洗脱，流速为1.0 mL·min^-1，柱温为30 ℃，检测波长为238 nm。结果马鞭草药材中马鞭草苷、戟叶马鞭草苷和毛蕊花苷的薄层色谱鉴别特征明显，专属性强；药材中马鞭草苷、戟叶马鞭草苷和毛蕊花苷的线性范围分别为0.05~1.26 μg(r=0.999 9)、0.10~2.58 μg(r=0.999 8)和0.09~2.20 μg(r=0.999 5)，平均回收率(n=9)分别为96.8%(RSD=1.15%)、98.3% (RSD=2.34%) 和 98.2% (RSD=2.28%)。结论所建立的马鞭草药材定性定量测定方法简单准确，能够为马鞭草药材的质量控制提供有效数据。

Abstract: OBJECTIVE To establish a method for quality control of Verbenae Herba. METHODS Verbenalin, hastatoside and acteoside in Verbena officinalis L. were identified by TLC. Their contents were determined by HPLC equipped with Agilent Eclipse XDB C₁₈ (4.6 mm×250 mm, 5 μm) column, the mobile phase consisted of acetonitrile-methanol-water, the anlysis was done at a flow rate of 1.0 mL·min^-1, the column temperature was 30 ℃ and the detection wavelength was 238 nm. RESULTS The linearities of verbenalin, hastatoside and acteoside were in the ranges of 0.05-1.26 μg(r=0.999 9), 0.10-2.58 μg(r=0.999 8) and 0.09-2.20 μg (r=0.999 5), respectively. The average recoveries (n=9) were 96.8% (RSD=1.15%) for verbenalin, 98.3% (RSD=2.34%) for hastatoside, and 98.2% (RSD=2.28%) for acteoside. CONCLUSION This method is simple, accurate with good reproducibility. It is suitable for quality control of Verbenae Herba.

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