Abstract: OBJECTIVE To compare the accumulative absorption of the phytosomes of baicalin and baicalin via in vitro small intestine. METHODS Everted intestinal sacs were used and the concentration of baicalin was determined by HPLC. RESULTS Within 120 min, the concentration of the phytosomes of baicalin and baicalin were increasing over the time; the accumulative absorption of the baicalin in vitro small intestine was obviously higher than that of the phytosomes of baicalin(P<0.05). CONCLUSION The accumulative absorption of the baicalin in small intestine was higher than the absorption of the phytosomes of baicalin, the main site that phytosomes of baicalin increased the absorption of baicalin was not in small intestine.