HPLC测定芹菜籽提取物中木犀草素含量

Determination of Luteolin in Celery Seed Extract by HPLC

摘要: 目的建立高效液相色谱法测定芹菜籽提取物中木犀草素的含量。方法采用Apollo C₁₈柱(4.6 mm×250 mm，5 μm)；流动相为乙腈-0.1%磷酸溶液(30∶70)；流速为1.0 mL·min^-1；进样量为20 μL；检测波长为269 nm；柱温为 30 ℃。结果木犀草素浓度线性范围为0.50~10.0 μg·mL^-1(r=0.999 8)，3个浓度的加样回收率分别为100.2%，100.1%和101.2%，RSD分别为1.02%，1.91%和1.75%。结论本法操作简单、准确、专属性强，可作为芹菜籽提取物质量控制的方法。

Abstract: OBJECTIVE To establish a method for the determination of luteolin in celery seed extract. METHODS The analysis was performed by HPLC with Apollo C₁₈ column (4.6 mm×250 mm, 5 μm). The mobile phase was consisted of acetonitrile-0.1% phosphoric acid (30∶70), and the detection wavelength was set at 269 nm. The column temperature was 30 ℃. RESULTS The linear range was 0.50 to 10.0 μg·mL^-1 for luteolin (r=0.999 8). The average recovery of three concentration were 100.2%, 100.1% and 101.2%, RSD were 1.02%, 1.91% and 1.75%. CONCLUSION The method is feasible, accurate and high selectivity for the determination of luteolin in celery seed extract.