Abstract: OBJECTIVE To study the effect of luteolin and baicalin mixture in purpose-made cigarette smoke on respiratory system. METHODS Purpose-made cigarette was made by adding luteolin and baicalin mixture into cigarette. Mixture in smoke contacted with respiratory system by smoking machine. The cough model induced by ammonia in mice was used. The effect of mixture on tracheal excretion was evaluated by phenol red excretion test in mice. The action of luteolin and baicalin mixture on asthma was investigated in histamine and acetylcholine induced-asthma guinea pig. Acute toxicity tests of oral luteolin and baicalin mixture and purpose-made cigarette were conducted. RESULTS Luteolin and baicalin mixture in smoke reduced the frequency of cough ammonia-induced in mouse, increased the tracheal excretion compared with control cigarette, and eased the incitement to respiratory system. CONCLUSION Luteolin and baicalin mixture in purpose-made cigarette smoke can diminish perniciousness of cigarette and improve the quality of cigarette in sensory evaluation to some extent.