HPLC测定棉油皂脚中棉酚含量

Determine of Gossypol in Cottonseed Soapstock by HPLC

摘要: 目的建立高效液相色谱法测定棉油皂脚中总棉酚(TGP)与游离棉酚(FGP)含量的方法。方法采用Waters Bondapak C₁₈ Column(3.9 mm×150 mm，4 μm)；流动相为乙腈-0.7%磷酸水溶液(80∶20)；流速为1.0 mL·min^-1；检测波长为238 nm；柱温为25 ℃。结果棉酚在10~60 μg·mL^-1(r=0.999 4)内线性关系良好，平均加样回收率为99.03%，RSD=1.72%(n=3)。结论本法简便、快速、准确、重复性高，可用于对棉油皂脚中棉酚的含量测定。

Abstract: OBJECTIVE To determine the content of total gossypol and liberation gossypol by HPLC. METHODS The analysis was performed on a Waters Bondapak C₁₈ Column(3.9 mm×150 mm, 4 μm) at 25 ℃ with acetonitrile-0.7% phosphoric acid water solution(80∶20) as the mobile phase and the flow rate of 1.0 mL·min^-1. The detection wavelength was set at 238 nm. RESULTS Gossypol have good linearity in the range of 10-60 μg·mL^-1(r=0.999 4). The average recovery was 99.03%, RSD=1.72%(n=3). CONCLUSION The developed method is convenient, rapid and accurate with high repeatability, which is suitable to assay the quality of gossypol.