HPLC同时测定大豆磷脂中磷脂酰胆碱和溶血磷脂酰胆碱含量

Determination of Phosphatidylcholine and Lysophosphatidylcholine in Soybean Phospholipids by HPLC

摘要: 目的建立同时测定大豆磷脂中磷脂酰胆碱(PC)和溶血磷脂酰胆碱(LPC)含量的方法。方法采用高效液相色谱法，色谱柱为Phenomenex silica柱(4.6 mm×150 mm，5 μm)，流动相为正己烷-异丙醇-水(5∶80∶20)，流速：0.7 mL·min^-1，检测波长：206 nm，柱温：25 ℃。结果 PC在0.4~2.4 mg·mL^-1内呈良好的线性关系，平均回收率为99.4%，RSD为0.40%(n=6)；LPC在10~60 μg·mL^-1内呈良好的线性关系，平均回收率为98.0%，RSD为1.65%(n=6)。结论本试验方法简便、重复性好、专属性强，可作为大豆磷脂中PC，LPC的检测方法。

Abstract: OBJECTIVE To establish an HPLC method for simultaneously determining phosphatidylcholine (PC), lysophosphatidylcholine (LPC) in soybean phospholipids. METHODS The HPLC system consisted of a Phenomenex silica column(4.6 mm×150 mm, 5 μm), a mobile phase of n-hexane-isopropanol-water(5∶80∶20). The flow rate was 0.7 mL·min^-1. Column temperature was set at 25 ℃ and the wavelength of detector was set at 206 nm. RESULTS The calibration curve was linear in the range of 0.4-2.4 mg·mL^-1 for PC and 10-60 μg·mL^-1 for LPC, respectively. The average recovery for PC was 99.4% and RSD was 0.40%(n=6). The average recovery for LPC was 98.0% and RSD was 1.65%(n=6). CONCLUSION The method is simple, rapid and specially. It can be used for the determination of PC and LPC in soybean phospholipids.