Abstract: OBJECTIVE To study the optimal enzyme extraction technology of polysaccharides from Polygoni Multiflori Caulis. METHODS The yield of polysaccharide and polysaccharide content were used to evaluate the factor levels. Based on the optimized enzyme, the extraction conditions were optimized by the single factor tests. RESULTS The conditions of the optimal extraction process using cellulose enzyme method were as follows: the pH value was 5.0, the concentration of cellulose enzyme was 3%, the extraction time was 4 hours and the extraction temperature was 55 ℃. CONCLUSION The yield of polysaccharide extracted from Polygoni Multiflori Caulis can be significantly increased by using cellulose enzyme extraction process.