Abstract: OBJECTIVE To study the optimum extraction process of polysaccharide from Hyssopus offcinalis. METHODS The optional ultrasonic extraction process was selected with the orthogonal design and the content of polysaccharides was determined by spectrophotometer. RESULTS λ_max=620 nm, RSD=2.84%(n=3), the optimum extraction process was as follows: the polysaccharide was extracted with ultrasonic technology for 1 hour with 25 times water for 2 times and alcohol concentration was 80%. CONCLUSION The experimental results provide the basis for the ultrasonic extraction process of polysaccharide from Hyssopus offcinalis. This optimum process is simple, stable and efficient.