HPLC测定九里香中脱水长叶九里香内酯的含量

Determination of Phebalosin in Murrayae Folium et Cacumen by HPLC

摘要: 目的建立九里香药材中脱水长叶九里香内酯的含量测定方法。方法采用高效液相色谱法，使用Agilent Esclipe XDB-C¹⁸色谱柱(150 mm×4.6 mm，5 μm)，流动相为甲醇-水(55∶45)，流速1 mL·min^-1，检测波长332 nm，柱温25 ℃。结果脱水长叶九里香内酯进样浓度在20.72~207.2 μg·mL^-1内，与峰面积呈良好的线性关系(r=0.999 8)；其平均回收率为98.41%，RSD为1.65%(n=6)。结论本法简便易行，重复性好，可用于九里香药材中脱水长叶九里香内酯的含量测定。

Abstract: OBJECTIVE To establish a high performance liquid chromatographic method for the determination of phebalosin in Murrayae Folium et Cacumen. METHODS The analysis was carried out on an Agilent Esclipe XDB-C18 column(150 mm×4.6 mm, 5 μm), using a mixture of 55% methanol and 45% water as the mobile phase at a flow rate of 1 mL·min^-1 at 25 ℃, the wavelength for measurement was 332 nm. RESULTS The calibration curve was linear in the range of 20.72-207.2 μg·mL^-1(r=0.999 8); the average recovery was 98.41% with RSD of 1.65%(n=6). CONCLUSION The method is simple, accurate and reproducible, and it is suitable for the determination of phebalosin in Murrayae Folium et Cacumen.