Abstract: OBJECTIVE To provide scientific basis for the utilization and development of Ziziphora Bungeana Herba by setting up the quality control specification of Ziziphora Bungeana Herba. METHODS In different origin of 6 batches of Ziziphora bungeana, the samples of the original plant were identificated, its properties were described. The stem, leaf, flower powder for microscopic were identificated in contrast to pulegone on TLC. The 6 batches of Ziziphora bungeana of moisture and ash were determined. The essential oil determination method of Ziziphora bungeana were inspected and the bioactive constituents were analyzed by HPLC. RESULTS The origins, macroscopic and microscopic identification of Ziziphora bungeana were described and the TLC identification method was formulated. Check in formulating moisture was less than 9%, total ashes were less than 10%, acid-insoluble ashes were less than 2%. Determine the essential oil and pulegone of Ziziphora bungeana and according to the content determination method for the determination of the essential oil product shall be not less than 0.60% (mL·g^-1), pulegone shall not be less than 0.30%. CONCLUSION The established method can be used for the quality control of Ziziphora Bungeana Hebra.