正交设计法优选穿山龙总皂苷的超声提取工艺

Orthogonal Design for Optimization of Ultrasonic Extracting Procedure for Total Saponins from Dioscoreae Nipponicae Rhizoma

摘要: 目的确定穿山龙总皂苷的最佳超声提取工艺。方法采用正交试验设计进行条件优选，通过分光光度法对提取物进行总皂苷含量的测定。结果检测波长为410 nm，薯蓣皂苷元在0.004~0.020 mg·mL^-1内呈良好的线性关系(r=0.999 8)，最佳超声提取工艺为：以水饱和正丁醇为提取剂，料液比1∶30，超声温度70 ℃，超声功率180 W。结论优选出的超声提取工艺合理、经济、可行。

Abstract: OBJECTIVE To determine the best ultrasonic extracting procedure for total saponins from Dioscoreae Nipponicae Rhizoma. METHODS Orthogonal experiment was used to optimize the condition. The content of total saponins was determined by spectrophotometric method. RESULTS The wavelength of the maximum absorption was 410 nm. Diosgenin showed a good linear relationship at the range of 0.004-0.020 mg·mL^-1(r=0.999 8). The optimum ultrasonic extraction conditions were water-saturated n-butanol as extraction agent, solid-liquid ratio 1∶30, temperature 70 ℃ and ultrasonic power 180 W. CONCLUSION The ultrasonic extracting procedure is reasonable, economical and feasible.