野菊花中总多糖提取条件的研究

Study on Extraction of Total Polysaccharides in Chrysanthemi Indici Flos

摘要: 目的研究野菊花总多糖的提取条件，并测定不同产地野菊花水煎剂中总多糖的含量。方法采用苯酚-硫酸法测定总多糖含量，并对多糖显色方式进行优选。结果野菊花中总多糖回收率为100.3%，RSD为1.19%，不同产地野菊花中总多糖含量存在差异。结论该方法准确、快速、重复性好，可用于测定野菊花中总多糖的含量。

Abstract: OBJECTIVE To study on extraction of total polysaccharides in Chrysanthemi Indici Flos. And determine total polysaccharides in water extraction of Chrysanthemi Indici Flos from various habitats. METHODS Total polysaccharides were determined with phenol-sulfuric acid method and orthogonal test was used to select the best coloration. RESULTS Recovery of total polysaccharides was 100.3%, RSD was 1.19%, there was difference among Chrysanthemi Indici Flos from various habitats. CONCLUSION The method is accurate, repeatable and suitable to determine total polysaccharides in water extraction of Chrysanthemi Indici Flos.