HPLC测定蒙药曼陀罗子中东莨菪碱的含量

Determination of Scopolamine in Datura stramonium by HPLC

摘要: 目的建立曼陀罗子中东莨菪碱的含量测定方法。方法色谱柱为Kromasil C₁₈(250 mm×4.6 mm，5 mm)。乙腈-水(含0.07 mol·L^-1磷酸钠和0.017 5 mol·L^-1十二烷基硫酸钠，0.5%磷酸，0.1%三乙胺)(37∶63)为流动相，检测波长为216 nm。结果东莨菪碱在0.038 1~0.190 5 mg·mL^-1内呈良好的线性，r=0.999 9；平均回收率为97.0%，RSD为0.93%。结论该方法简便、灵敏、准确、重复性好，可作为曼陀罗子的质量控制标准。

Abstract: OBJECTIVE To establish the method of determination of scopolamine in Datura stramonium. METHODS Kromasil C₁₈(250 mm×4.6 mm, 5 mm) column was employed. Mobile phase was the mixture of acetonitrile and Na3PO4 buffer (contains 0.07 mol·L^-1 Na₃PO₄, 0.017 5 mol·L^-1 SDS, 0.5% H₃PO₄, 0.1% trithylamine)(37∶63). RESULTS A good linearity of scopolamine was achieved in the range of 0.038 1-0.190 5 mg·mL^-1(r=0.999 9). The average recovery of scopolamine was 96.97% and RSD was 0.93%. CONCLUSION This method is simple, sensitive, accurate with good reproducibility, and can be used for the quality control of Datura stramonium.