鱼皮胶原蛋白水解液中小分子寡肽的分子量分布测定

Determination of the Molecular Weight Distribution of Small Molecule Oligopeptide in Fish Skin Collagen Hydrolyzate

摘要: 目的建立测定鱼皮胶原蛋白水解液中小分子寡肽相对分子量分布的方法。方法采用高效凝胶排阻色谱法用3根TSK-GEL G2000SWXL色谱柱(300 mm×7.8 mm，5 μm)串联，室温；流动相为乙腈-0.1%三氟乙酸溶液(15∶85)，流速0.6 mL·min^-1，检测波长220 nm。结果在相对分子量149~2 490 Da内，寡肽相对分子量的对数与其保留时间具有良好的线性关系(r=0.995 6)；精密度和重复性RSD均<0.1%。结论本方法简便准确，重复性好，可作为测定小分子寡肽分子量分布的方法。

Abstract: OBJECTIVE To establish a method for determining the molecular weight distribution of small molecule oligopeptide in fish skin collagen hydrolyzate. METHODS The molecular weight distribution was determined using three TSK-GEL G 2000SWXL columns (300 mm×7.8 mm, 5 μm) in series at room temperature with the mobile phase of acetonitrile-0.1% trifluoracetic acid solution (15∶85) at the flow rate of 0.6 mL·min^-1. The detection wavelength was 220 nm. RESULTS In the molecular weight range of 149-2 490 Da, there was a good linear relationship between the logarithm of molecular weight of oligopeptide and the retention time(r=0.995 6). The RSD of precision and repeatability were both less than 0.1%. CONCLUSION The method is simple, accurate and reproducible for the determination of the molecular weight distribution of small molecule oligopeptide.