葛根素衍生物抗炎作用及对环氧化酶活性的影响

Affects on Cyclooxygenase Activities and Anti-inflammations Effects of Tetra-acetylated Puerarin

摘要: 目的探讨四乙酰葛根素(4Ac)的抗炎作用以及对环氧化酶(COX)的抑制作用。方法采用角叉菜胶诱发的大鼠急性足肿胀模型进行抗炎作用研究，并对环氧化酶的选择性进行了研究。结果 4Ac(40 mg·kg^-1)对模型大鼠的足肿胀均有明显的抑制作用；4Ac对COX-2活性的半数抑制浓度(IC₅₀)约为52.9 μmol·L^-1，与吲哚美辛相比，无统计学差异(P>0.05)。

Abstract: OBJECTIVE To investigate anti-inflammatory effects and cyclooxygenase-inhibitory effects of tetra-acetylated puerarin. METHODS The anti-inflammatory effect of tetra-acetylated puerarin was observed by using the rat inflammation model induced by Carrageenan, and the cyclooxygenase activity of tetra-acetylated puerarin was observed by radioimmunoassay(RIA). RESULTS Tetra-acetylated puerarin (40 mg·kg^-1) can significantly decrease the rat paw edema for the inhibition effects on foot edema. Compared with indomethacin, the effect on COX-2 of puerarin derivatives showed no significant difference(P>0.05). And the 50% inhibitory concentration (IC_{50-1. CONCLUSION Tetra-acetylated puerarin has significant anti-inflammatory activity and selective inhibition effect on the COX-2, and it has a good application prospects.}