Abstract: OBJECTIVE To get a stable artemisinin liposome with high trapping efficiency by selecting formulation and preparation of artemisinin liposome. METHODS The artemisinin liposomes were prepared by film-ultrasound method. The formulation was optimized by orthogonal design. The trapping efficiency and the stability were determined. RESULTS The trapping efficiency of the artemisinin liposome reached 85%, the drug amounted to 27.22%, the particle size of the liposome was 90 nm and the Zeta potential was -68.4 mV. The liposome had good stability. CONCLUSION The method can be used to prepare a stable artemisinin liposome with high trapping efficiency.