基于分析质量源于设计理念同时测定黄酒中14种酚酸及黄酮类成分

戴泰宇; 赵敏彤; 颉佳乐; 刘镇; 胡银洲; 陆晓燕; 龚行楚

doi:10.13748/j.cnki.issn1007-7693.20252575

基于分析质量源于设计理念同时测定黄酒中14种酚酸及黄酮类成分

Simultaneous Determination of 14 Phenolic Acids and Flavonoids in Huangjiu Based on the Analytical Quality by Design

摘要

摘要:
目的基于分析质量源于设计(analytical quality by design，AQbD)理念建立同时测定黄酒中14种酚酸及黄酮类成分的高效液相色谱-二极管阵列检测器(HPLC-DAD)定量分析方法。
方法采用确定性筛选设计对柱温、流量及梯度洗脱程序等6个因素进行优化，考察其对关键方法属性(分离度与出峰时间)的影响，构建定量模型，并运用蒙特卡洛法计算方法可操作设计区域。
结果方法学验证结果表明，各成分线性关系良好(R²≥0.9994)，精密度、稳定性及重复性RSD均<2.0%，平均加样回收率为92.3%~104.7%。应用所建方法测定10批次黄酒样品中多酚含量，结果显示香草酸含量显著高于其他成分。
结论建立的方法准确、可靠，适用于黄酒中酚酸和黄酮类成分的定量分析，为其质量控制和健康功能评价提供技术支撑。

Abstract:
OBJECTIVE To establish a high-performance liquid chromatography-diode array detector(HPLC-DAD) method based on analytical quality by design(AQbD) for simultaneous determination of 14 phenolic acids and flavonoids in Huangjiu.
METHODS Six critical factors including column temperature and gradient elution program were optimized using definitive screening design(DSD). Quantitative models were established, and the method operable design region was calculated using the Monte Carlo method.
RESULTS Method validation demonstrated good linearity for all analytes(R²≥0.9994), with RSDs for precision, stability, and repeatability all below 2.0%, and average recoveries ranging from 92.3% to 104.7%. The established method was applied to determine polyphenol contents in 10 batches of Huangjiu samples, and the results showed that vanillic acid was present at significantly higher levels than the other polyphenols.
CONCLUSION The developed method is accurate and reliable, providing effective technical support for quality control and health function assessment of Huangjiu.

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