Abstract: Human epidermal growth factor(hEGF) is a crucial growth factor widely applied in wound healing, skin repair, tissue engineering and other fields. In recent years, advances in gene recombinant technology have driven the efficient production of hEGF. Researchers have successfully optimized hEGF expression using both prokaryotic expression systems(e.g., Escherichia coli, Bacillus, Lactobacillus) and eukaryotic expression systems(e.g., fungi, insect cells, mammalian cells, plant cells), laying the foundation for its large-scale production. Furthermore, the diversified development of hEGF formulations has opened up more possibilities for its clinical applications. This review summarizes research progress on hEGF across different expression systems and formulation applications, aiming to provide reference for its further clinical utilization.