Abstract:
OBJECTIVE To establish an effective method to rapidly identify the chemical components of the classic Chinese medicine prescription Yigan San, and to explore its migratory components in blood and brain tissues after oral administration.
METHODS Data acquisition was performed by ultra-performance liquid chromatography-quadrupole-time-of-flight tandem mass spectrometry(UHPLC-Q-TOF-MS/MS). According to the information of compound retention time, excimer ion peak, secondary fragment ion and other information, the migration components in blood and brain tissues after oral administration were characterized and identified.
RESULTS A total of 170 compounds were identified from the water extract of Yigan San, including 42 flavonoids, 33 others, 25 lactones, 27 organic acids, 14 terpenoids, 11 alkaloids, 10 coumarins and 8 saponins. A total of 31 compounds were identified in the blood and 8 compounds in the brain tissue. Additionally, 13 metabolites were discovered, covering phase Ⅰ and phase Ⅱ metabolic processes such as reduction, oxidation, hydroxylation, and glucuronidation.
CONCLUSION In this study, the chemical composition of Yigan San are analysed in high throughput, revealing its delivery in blood and brain tissues after oral administration, in which scopoletin, senkyunolide, and formononetin may be the basis of potential pharmacodynamic substances, which may provide a scientific basis for the improvement of the quality standard of the compound, the study of pharmacodynamic substances, and the development of the active pharmaceuticals in the future.
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