OBJECTIVE  To verify the feasibility of using real-time fluorescent quantitative PCR(qPCR) for rapid detection as an alternative to the post-enrichment detection method for Burkholderia cepacia complex(Bcc) in pharmaceutical water.

METHODS Firstly, a comparative study on the enrichment efficacy of undiluted tryptic soy broth(TSB) medium and 10-fold diluted TSB medium for Burkholderia species was conducted using 35 strains of Burkholderia microorganisms. Subsequently, a fully automated nucleic acid amplification detection system based on qPCR technology was employed to systematically validate the specificity, limit of detection(LOD), robustness, and reproducibility of the alternative method for post-enrichment detection of Bcc in pharmaceutical water.

RESULTS  Ten-fold diluted TSB medium was superior to the undiluted TSB medium for the enrichment of Bcc strains within the Burkholderia genus. The LOD of the alternative method in this study was consistent with that of the pharmacopoeia culture method, and the results for specificity, robustness, and reproducibility were all satisfactory.

CONCLUSION The 10-fold diluted TSB medium is more suitable for the enrichment culture of Bcc strains within the Burkholderia genus. qPCR is suitable for rapid detection of Bcc in pharmaceutical water after enrichment culture.