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    芳香烃受体和雄激素受体对HepG2细胞中脂质代谢的影响

    Effects of Aryl Hydrocarbon Receptor and Androgen Receptor on Lipid Metabolism in HepG2 Cells

      摘要
    • 摘要:
      目的 探究芳香烃受体(aryl hydrocarbon receptor，AhR)和雄激素受体(androgen receptor，AR)对HepG2细胞中脂质代谢的影响。
      方法 将HepG2细胞分为空白对照(Control)组、6-甲酰基吲哚并3,2-B咔唑(6-formylindolo3,2-b carbazole，FICZ)(AhR激动剂)组、二氢睾酮(dihydrotestosterone， DHT)(AR激动剂)组、FICZ+DHT组，按分组分别给予HepG2细胞1 μmol·L−1 FICZ及1 μmol·L−1DHT。试剂盒检测TG、TC和 LDL-C含量，油红O染色法检测脂质含量，尼罗红染色法检测脂质荧光强度。Western blotting检测胆固醇合成相关蛋白3-羟基-3-甲基戊二酸还原酶(3-hydroxy-3-methylglutaryl coenzyme A reductase，HMGCR)、固醇调节元件结合蛋白2(sterol regulatory element-binding protein 2，SREBP2)、肝X受体α(liver X receptor alpha，LXRα)，以及脂肪酸合成相关蛋白CCAAT增强子结合蛋白α(CCAAT enhancer binding protein alpha，C/EBPα)、过氧化物酶体增殖物激活受体γ(peroxisome proliferator-activated receptor γ，PPARγ)和硬脂酰辅酶A去饱和酶1(stearoyl-CoA desaturase 1，SCD1)的表达情况。
      结果 与Control组相比，FICZ组HepG2细胞的脂质含量升高，而DHT组则降低；与FICZ组相比，FICZ+DHT组HepG2细胞中脂质含量降低。与Control组相比，FICZ组HMGCR、SREBP2、LXRα蛋白表达增加，而DHT组则减少；与FICZ组相比，FICZ+DHT组HMGCR、SREBP2、LXRα蛋白表达减少。与Control组相比，FICZ组SCD1、C/EBPα、PPARγ蛋白表达增加，而DHT组则减少；与FICZ组相比，FICZ+DHT组SCD1、C/EBPα、PPARγ蛋白表达减少。
      结论 AhR和AR存在相互作用，对脂质代谢的影响呈相互拮抗作用。

       

      Abstract:
      OBJECTIVE To investigate the effects of aryl hydrocarbon receptor(AhR) and androgen receptor(AR) on lipid metabolism in HepG2 cells.
      METHODS HepG2 cells were divided into Control group, 6-formylindolo3,2-bcarbazole(FICZ, AhR agonist) group, dihydrotestosterone(DHT, AR agonist) group, and FICZ+DHT group. HepG2 cells were given 1 μmol·L−1 FICZ and 1 μmol·L−1 DHT according to the groups. Kits were used to detect the levels of TG, TC and LDL-C. Oil red O staining was used to detect lipid content, and Nile Red staining was used to detect lipid fluorescence intensity. Western blotting was used to detect the expression of cholesterol synthesis-related proteins3-hydroxy-3-methylglutaryl coenzyme A reductase(HMGCR), sterol regulatory element-binding protein 2(SREBP2), liver X receptor alpha(LXRα) and fatty acid synthesis-related proteinsCCAAT enhancer binding protein alpha(C/EBPα), peroxisome proliferator-activated receptor γ(PPARγ) and stearoyl-CoA desaturase 1(SCD1).
      RESULTS Compared with the Control group, lipid content in HepG2 cells increased in the FICZ group but decreased in the DHT group. Compared with the FICZ group, the lipid content in HepG2 cells in the FICZ+DHT group decreased. Compared with the Control group, the expression of HMGCR, SREBP2 and LXRα proteins increased in the FICZ group but decreased in the DHT group. Compared with the FICZ group, the expression of HMGCR, SREBP2 and LXRα proteins in the FICZ+DHT group decreased. Compared with the Control group, the expression of SCD1, C/EBPα and PPARγ proteins increased in the FICZ group but decreased in the DHT group. Compared with the FICZ group, the expression of SCD1, C/EBPα, and PPARγ proteins in the FICZ+DHT group decreased.
      CONCLUSION AhR and AR interact with each other and their effects on lipid metabolism are antagonistic.

       

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