UHPLC-Q-TOF-MS同时测定铁皮石斛中2个专属性黄酮苷的含量

罗镭; 黄岚; 陈碧莲

doi:10.13748/j.cnki.issn1007-7693.20241496

UHPLC-Q-TOF-MS同时测定铁皮石斛中2个专属性黄酮苷的含量

Simultaneous Determination of 2 Specific Flavonoids Glycosides in Dendrobium Officinale by UHPLC-Q-TOF-MS

摘要

摘要:
目的建立液质联用(UHPLC-Q-TOF-MS)同时测定铁皮石斛中apigenin-6-C-(2′′-O-β-D-glucopyranosyl)-α-L-arabinopyranoside和apigenin-8-C-(2′′-O-β-D-glucopyranosyl)-α-L-arabinopyranoside含量的方法。
方法采用Agilent ZORBAX SB C₁₈(2.1 mm×100 mm，1.8 µm)色谱柱，以乙腈(A)- 0.1%甲酸水(B)为流动相进行梯度洗脱，柱温为30 ℃，流速为0.25 mL·min⁻¹，进样量为2 µL。采用电喷雾双喷离子源(Dual AJS ESI)，负离子模式，质量扫描范围为m/z100~1700。
结果 Apigenin-6-C-(2′′-O-β-D-glucopyranosyl)-α-L-arabinopyranoside和apigenin-8-C-(2′′-O-β-D-glucopyranosyl)-α-L-arabinopyranoside分别在0.00496~0.248 μg·mL⁻¹(r=0.999 9)、0.01079~0.5379 μg·mL⁻¹(r=0.999 9) 内呈良好线性关系，平均加样回收率分别为99.04%、100.41%；RSD分别为2.09%、2.00%。
结论本方法简便、快速、灵敏度高、专属性好，可为铁皮石斛药材的质量控制提供参考依据。

Abstract:
OBJECTIVE To establish an UHPLC-Q-TOF-MS method for the simultaneous determination of apigenin-6-C-(2′′-O-β-D-glucopyranosyl)-α-L-arabino pyranoside and apigenin-8-C-(2′′-O-β-D-glucopyranosyl)-α-L-arabinopyranoside in Dendrobium officinale.
METHODS The separation was performed on an Agilent ZORBAX SB C₁₈(100 mm×2.1 mm, 1.8 µm)column with a mobile phase consisting of acetonitrile(A) and 0.1% formic acid water solution(B) with gradient elution at a flow rate of 0.25 mL·min⁻¹, the column temperature was 30 ℃ and the injection volume was 2 µL. The MS spectrometry detection was performed with electrospray ionization in negative mode, the scanning rang was m/z 110–1700.
RESULTS Apigenin-6-C-(2′′-O-β-D-glucopyranosyl)-α-L-arabinopyranoside and apigenin-8-C-(2′′-O-β-D-glucopyranosyl)-α-L-arabinopyranoside were showed good linear relationships within the ranges of 0.00496–0.248 μg·mL⁻¹(r=0.999 9) and 0.01079–0.5379 μg·mL⁻¹(r=0.999 9). The average recoveries were 99.04% and 100.41%, RSD were 2.09% and 2.00%, respectively.
CONCLUSION This method is simple, rapid, sensitive, with good specificity, and it can provide a reference for the quality control of Dendrobium officinale.

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