葶苈子中抗心肌细胞凋亡成分筛选及机制研究

李静阳; 张莉; 杨方方; 李孟; 夏明洋; 张礼轩; 冯卫生; 郑晓珂

doi:10.13748/j.cnki.issn1007-7693.20241494

葶苈子中抗心肌细胞凋亡成分筛选及机制研究

Screening of Anti-cardiomyocyte Apoptosis Components in Descurainiae Semen Lepidii Semen and Study of Mechanism

摘要

摘要:
目的从葶苈子中分离得到42种化合物，并筛选对H₂O₂诱导的心肌细胞损伤模型具有改善作用的成分，初步探讨有效化合物的作用机制。
方法以200 μmol·L⁻¹ H₂O₂建立H9c2心肌细胞损伤模型，用葶苈子中42种化合物分别处理细胞6 h，检测细胞活力、氧化应激水平、凋亡水平和凋亡通路关键蛋白表达水平。
结果葶苈子中有10种化合物可以有效提升心肌细胞活力。其中，山柰酚-3-O-β-D-吡喃葡萄糖苷(DS-1)、槲皮素-3-O-β-D-吡喃木糖苷(DS-2)和北葶新苷A(LS-19)效果最好，它们可降低心肌细胞凋亡率，升高T-SOD、GSH-PX水平，降低LDH、MDA水平，改善氧化应激；提高线粒体膜电位水平，降低ROS水平，抑制细胞凋亡；降低Bax/Bcl-2，抑制凋亡通路关键蛋白Caspase-3表达水平。
结论葶苈子中化合物DS-1、DS-2和LS-19对H₂O₂诱导的H9c2细胞损伤具有显著保护作用，机制可能与改善氧化应激水平，抑制细胞凋亡有关。

Abstract:
OBJECTIVE To screen components with ameliorative effects on H₂O₂-induced cardiomyocyte injury from 42 compounds isolated from Descurainiae Semen Lepidii Semen and initially to investigate the mechanism of action of the effective compounds.
METHODS The H9c2 cardiomyocyte injury model was induced by 200 μmol·L⁻¹ H₂O₂, and the cells were treated with 42 compounds from Descurainiae Semen Lepidii Semen for 6 h, then measured cell viability, oxidative stress, apoptosis levels and expression of key proteins of the apoptosis pathway.
RESULTS Ten compounds in Descurainiae Semen Lepidii Semen could effectively enhance cell viability. Among them, Kaempferol-3-O-β-D-glucopyranoside (DS-1), quercetin-3-O-β-D-xylopyranoside (DS-2) and lepidiumoside A (LS-19) were the most effective, and they can reduce the apoptosis rate of cardiomyocyte, enhance T-SOD, GSH-PX levels, decrease LDH, MDA levels, improve oxidative stress, enhance mitochondrial membrane potential level, decrease ROS level, and inhibit apoptosis of cardiomyocytes, reduce the level of Bax/Bcl-2, and inhibit the expression level of the key protein Caspase-3 of the apoptosis pathway.
CONCLUSION DS-1, DS-2 and LS-19 from Descurainiae Semen Lepidii Semen have significant protective effect on H9c2 cardiomyocyte in H₂O₂-induced injury, the mechanism may be related to improve the level of oxidative stress homeostasis the and inhibit the apoptotic pathway.

HTML全文

参考文献(40)

施引文献

资源附件(0)