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    载隐丹参酮普鲁士蓝纳米粒的制备及评价

    Preparation and Evaluation of Cryptotanshinone-containing Prussian Blue Nanoparticles

      摘要
    • 摘要:
      目的 制备载隐丹参酮普鲁士蓝纳米粒 (cryptotanshinone-containing prussian blue nanoparticles，CPT@PB NPs)，并对制备得到的纳米粒的理化性质、光热性能及细胞毒性进行评价。
      方法 采用水热合成法和溶剂吸附法制备CPT@PB NPs；采用动态光散射技术和透射电子显微镜对纳米粒的粒径和形态进行表征；采用红外光热成像仪检测纳米粒的光热效应；最后，通过CCK-8法评估CPT@PB NPs的体外抗肿瘤活性。
      结果 制备的CPT@PB NPs呈规则立方体，具有明显的孔道结构，CPT的载药量为(1.80±0.23)%。CPT@PB NPs具有良好的光热性能，光热效率(η)为35.42%。CCK-8实验表明，激光照射下低浓度CPT@PB NPs即对4T1细胞表现出良好的杀伤作用，且光热疗法和化疗在抗肿瘤过程中发挥协同增效的作用。
      结论 CPT@PB NPs的成功制备为乳腺癌的治疗提供了新的研究方向。

       

      Abstract:
      OBJECTIVE To prepare cryptotanshinone-containing prussian blue nanoparticles(CPT@PB NPs) and evaluate the formulation's physicochemical properties, photothermal performance, and cytotoxicity.
      METHODS Hydrothermal synthesis method coupled with solvent adsorption technique was employed to obtain CPT@PB NPs. The particle size and morphology of nanoparticles were characterized by the dynamic light scattering and the transmission electron microscopy. The photothermal performance of nanoparticles was assessed using an infrared thermal imaging camera. Finally, the in vitro anti-tumor activity of CPT@PB NPs was evaluated by CCK-8 assay.
      RESULTS The synthesized CPT@PB NPs presented a regular cubic morphology with distinct porous structures. The drug loading of CPT was (1.80±0.23)%. The CPT@PB NPs exhibited excellent photothermal properties, with a photothermal conversion efficiency(η) of 35.42%. The CCK-8 assay indicated that the low concentration of CPT@PB NPs under laser irradiation had a significant cytotoxic effect on 4T1 cells, among which photothermal therapy and chemotherapy played synergistic roles in the anti-tumor process.
      CONCLUSION The successful preparation of CPT@PB NPs provides a new research direction for the treatment of breast cancer.

       

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