Abstract:
OBJECTIVE To analyze the chemical components of Cardiospermum halicacabum L. using UPLC-Q/TOF-MS/MS and establish a method for simultaneous determination of six flavonoid components in Cardiospermum halicacabum L. from different regions using HPLC.
METHODS UPLC-Q/TOF-MS/MS was performonced on Cortec UPLC C18 column(2.1 mm×100 mm, 1.6 μm) with mobile phase of methanol(A)-0.1% formic acid water(B) in gradient elution, with the flow rate of 0.2 mL·min−1, the column temperature of 35 ℃, injection volume of 2 μL; electrospray ionization(ESI) source was used to acquire mass spectrometry data in positive and negative ion modes with the scanning range of m/z 50–1 000. HPLC was performed on Shim-pack GIST C18(4.6 mm×250 mm, 5 μm) with mobile phase consisting of methanol(A) -0.1% formic acid water(B) in gradient elution, with the flow rate of 1 mL·min−1, column temperature of 35 ℃, detection wavelength of 350 nm, and injection volume of 10 μL. Principal component analysis(PCA) was conducted using SIMCA-P 14.1, and the components with significant differences(VIP>1) were screened.
RESULTS Utilizing the UPLC-Q/TOF-MS/MS technique, a total of 33 compounds were identified in both positive and negative ion modes. Based on the HPLC method, six flavonoid components were identified and PCA was conducted. The results of PCA showed that the Cardiospermum halicacabum L. from Fujian, Guangdong and Guangxi were grouped into one class respectively. The primary differential components in different batches of Cardiospermum halicacabum L. were luteolin-7-O-glucuronide, isoquercetin, and apigenin-7-O-glucuronide. Significant variations were observed among samples from different regions, while there was little difference between the samples of Cardiospermum halicacabum L. from Guangxi, and the quality was stable.
CONCLUSION Based on the UPLC-Q-TOF-MS/MS technique, rapid identification of chemical components of Cardiospermum halicacabum L. is realized. The established HPLC method for the determination of 6 flavonoids in Cardiospermum halicacabum L. is stable and reliable, which can provide reference for quality control.