基于UPLC-Q-Exactive-Orbitrap MS/MS的白豆蔻水提物化学成分及入血成分分析

巴达拉胡; 吴杰斯; 包白音木其尔; 刘小伟; 敖道夫

doi:10.13748/j.cnki.issn1007-7693.20233045

基于UPLC-Q-Exactive-Orbitrap MS/MS的白豆蔻水提物化学成分及入血成分分析

Analysis of Chemical Constituents and Components Absorbed into Blood of Baidoukou Aqueous Extract Based on UPLC-Q-Exactive-Orbitrap MS/MS

摘要

摘要:
目的采用UPLC-Q-Exactive-Orbitrap MS/MS技术，对白豆蔻水提物主要化学成分及大鼠口服入血成分进行分析。
方法采用ACQUITY UPLC HSS T3(2.1×100 mm，1.8 μm)色谱柱；以A(去离子水，含0.1%甲酸)和B(乙腈，含0.1%甲酸)为流动相，梯度洗脱，柱温：35 ℃；进样体积：10 μL；流速：0.2 mL·min⁻¹。采用电喷雾离子源(ESI)，Full MS/dd-MS²模式检测。
结果从白豆蔻水提物中共鉴定70种成分，包括有机酸类、黄酮类、氨基酸类、生物碱类、内酯类等。含药血清样本中分析19种原型成分，包括青蒿酸、壬二酸等10个有机酸类、异土木香内酯、去氢木香内酯等2种内酯类、甜菜碱、黄嘌呤等2种生物碱类、酪氨酸、色氨酸等3种氨基酸。
结论本研究首次阐明了白豆蔻水提物化学成分及其口服入血成分，为其进一步研究与开发提供科学的理论依据。

Abstract:
OBJECTIVE To analyze the main components of Baidoukou aqueous extract and the components that enter the blood of rats after oral administration by UPLC-Q-Exactive-Orbitrap MS/MS technology.
METHODS Using ACQUITY UPLC HSS T3 (2.1×100 mm, 1.8 μm) column; Using A(deionized water, containing 0.1% formic acid) and B (acetonitrile, containing 0.1% formic acid) as mobile phases, gradient elution, column temperature: 35°C; injection volume: 10 μL; flow rate: 0.2 mL·min⁻¹. Using electrospray ionization source (ESI), detect with Full ms/dd-ms² mode.
RESULTS A total of 70 components were identified from the aqueous extract of Baidoukou, including organic acids, flavonoids, amino acids, alkaloids, and lactones. 19 prototype components were identified in the drug-containing serum samples, including 10 organic acids such as artemisinic acid and azelaic acid, 2 lactones such as isoalantolactone and dehydrocostus lactone, 2 alkaloids such as Betaine and Xanthine, 3 amino acids such as L-Tyrosine and L-Tryptophan.
CONCLUSION It’s sclarified for the first time the chemical composition of Baidoukou aqueous extract and its oral blood components in this study, providing a theoretical basis for its further research and development.

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