基于UPLC-Q-TOF-MS/MS的银线莲化学成分分析

杨红霞; 许文; 黄泽豪

doi:10.13748/j.cnki.issn1007-7693.20232655

基于UPLC-Q-TOF-MS/MS的银线莲化学成分分析

Analysis of Chemical Constituents of Goodyerae Schlechtendalianae Herba Based on UPLC-Q-TOF-MS/MS

摘要

摘要:
目的采用超高效液相色谱-四极杆飞行时间质谱联用技术(UPLC-Q-TOF-MS/MS)快速鉴定银线莲中的化学成分。
方法采用Cortecs UPLC C₁₈(2.1 mm×100 mm，1.6 μm)色谱柱，流动相为甲醇-0.1%甲酸水，梯度洗脱，流速为0.25 mL·min⁻¹，柱温45 ℃。飞行时间质谱采用正负离子模式扫描。
结果根据UPLC-Q-TOF-MS/MS法推断鉴定出银线莲中28个化学成分，包括黄酮类成分7个，酚类成分15个，2-异丁基苹果酸葡萄糖氧基苄酯类成分4个，其他苷类成分2个。
结论 UPLC-Q-TOF-MS/MS技术为鉴定银线莲中化学成分提供了一种快速、高效的定性分析方法，研究结果可为银线莲的物质基础及质量标准研究提供理论依据。

Abstract:
OBJECTIVE To identify the chemical constituents in Goodyerae Schlechtendalianae Herba by UPLC-Q-TOF-MS/MS.
METHODS Cortecs UPLC C₁₈(2.1 mm×100 mm, 1.6 μm ) column was used. The mobile phase was methanol-0.1% formic acid with gradient elution. The flow rate was 0.25 mL·min⁻¹ and the column temperature was 45℃. MS monitoring was performed by electrospray ionization in positive and negative ion switching mode.
RESULTS Twenty-eight chemical constituents in Goodyerae Schlechtendalianae Herba were identified, including seven flavonoids, fifteen phenols, four 2-isopropyl-(glucosyloxy) benzyl malat and two other glycosides.
CONCLUSION UPLC-Q-TOF-MS/MS provides a rapid and efficient qualitative analysis method for the identification of chemical constituents in Goodyerae Schlechtendalianae Herba. The research results can provide a theoretical basis for the material basis and quality standard research of Goodyerae Schlechtendalianae Herba.

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